{"id":11563,"date":"2025-12-29T14:53:15","date_gmt":"2025-12-29T13:53:15","guid":{"rendered":"https:\/\/www.dentalcadmos.com\/?p=11563"},"modified":"2025-12-29T14:53:15","modified_gmt":"2025-12-29T13:53:15","slug":"gingival-fibroblast-proliferation-and-interaction-with-different-nanohybrid-resin-based-composite-materials-an-in-vitro-study","status":"publish","type":"post","link":"https:\/\/www.dentalcadmos.com\/en\/gingival-fibroblast-proliferation-and-interaction-with-different-nanohybrid-resin-based-composite-materials-an-in-vitro-study\/","title":{"rendered":"Gingival fibroblast proliferation and interaction with different nanohybrid resin-based composite materials: an in vitro study"},"content":{"rendered":"<p style=\"text-align: justify;\"><strong>OBJECTIVES:<\/strong> To evaluate the interactions between human gingival fibroblasts (hGFs) and resin-based composite materials of different viscosities by analyzing fibroblast proliferation, adhesion and overall cellular response.<\/p>\n<p style=\"text-align: justify;\"><strong>MATERIALS AND METHODS:<\/strong> Two nanohybrid composite resins were tested: Estelite Universal Flow (flowable) and Estelite ASTERIA (packable). Cylindrical specimens were prepared, sterilized and incubated with hGFs under controlled conditions. Cells were seeded at different densities and examined using LIVE&amp;DEAD staining at 24 hours, 3 days and 7 days to evaluate cell viability, morphology and proliferation. The experimental setup followed ISO 10993-12:2012 guidelines for cytotoxicity testing.<\/p>\n<p style=\"text-align: justify;\"><strong>RESULTS:<\/strong> The packable composite showed superior biocompatibility, with higher cell viability and proliferation than the flowable composite. After 3 days, cells on the packable material exhibited enhanced scaffold colonization and maintained fibroblast-like morphology, whereas the flowable composite showed delayed organization and reduced colonization. A significant reduction in cell viability was observed after 7 days, particularly in the flowable group, likely due to cumulative cytotoxic effects from monomer release.<\/p>\n<p style=\"text-align: justify;\"><strong>CONCLUSIONS:<\/strong> Both composite resins demonstrated acceptable biocompatibility, although differences emerged based on viscosity and composition. The packable composite exhibited better performance in supporting cell proliferation and viability.<\/p>\n<p style=\"text-align: justify;\"><strong>CLINICAL SIGNIFICANCE:<\/strong> Material selection, particularly viscosity and monomer composition, may influence soft-tissue behavior around restorations, highlighting the importance of choosing composites with favorable biological profiles in everyday clinical practice.<\/p>\n<p><i>Per continuare la lettura gli abbonati possono scaricare l\u2019allegato<\/i><\/p>\n","protected":false},"excerpt":{"rendered":"<p>OBJECTIVES: To evaluate the interactions between human gingival fibroblasts (hGFs) and resin-based composite materials of different viscosities by analyzing fibroblast proliferation, adhesion and overall cellular response. MATERIALS AND METHODS: Two nanohybrid composite resins were tested: Estelite Universal Flow (flowable) and Estelite ASTERIA (packable). Cylindrical specimens were prepared, sterilized and incubated with hGFs under controlled conditions. &hellip; <a href=\"https:\/\/www.dentalcadmos.com\/en\/gingival-fibroblast-proliferation-and-interaction-with-different-nanohybrid-resin-based-composite-materials-an-in-vitro-study\/\">Continued<\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v17.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Gingival fibroblast proliferation and interaction with different nanohybrid resin-based composite materials: an in vitro study - Dental Cadmos<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.dentalcadmos.com\/en\/gingival-fibroblast-proliferation-and-interaction-with-different-nanohybrid-resin-based-composite-materials-an-in-vitro-study\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Gingival fibroblast proliferation and interaction with different nanohybrid resin-based composite materials: an in vitro study - Dental Cadmos\" \/>\n<meta property=\"og:description\" content=\"OBJECTIVES: To evaluate the interactions between human gingival fibroblasts (hGFs) and resin-based composite materials of different viscosities by analyzing fibroblast proliferation, adhesion and overall cellular response. MATERIALS AND METHODS: Two nanohybrid composite resins were tested: Estelite Universal Flow (flowable) and Estelite ASTERIA (packable). Cylindrical specimens were prepared, sterilized and incubated with hGFs under controlled conditions. &hellip; Continued\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.dentalcadmos.com\/gingival-fibroblast-proliferation-and-interaction-with-different-nanohybrid-resin-based-composite-materials-an-in-vitro-study\/\" \/>\n<meta property=\"og:site_name\" content=\"Dental Cadmos\" \/>\n<meta property=\"article:published_time\" content=\"2025-12-29T13:53:15+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"edra\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.dentalcadmos.com\/#website\",\"url\":\"https:\/\/www.dentalcadmos.com\/\",\"name\":\"Dental Cadmos\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.dentalcadmos.com\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.dentalcadmos.com\/gingival-fibroblast-proliferation-and-interaction-with-different-nanohybrid-resin-based-composite-materials-an-in-vitro-study\/#webpage\",\"url\":\"https:\/\/www.dentalcadmos.com\/gingival-fibroblast-proliferation-and-interaction-with-different-nanohybrid-resin-based-composite-materials-an-in-vitro-study\/\",\"name\":\"Gingival fibroblast proliferation and interaction with different nanohybrid resin-based composite materials: an in vitro study - Dental Cadmos\",\"isPartOf\":{\"@id\":\"https:\/\/www.dentalcadmos.com\/#website\"},\"datePublished\":\"2025-12-29T13:53:15+00:00\",\"dateModified\":\"2025-12-29T13:53:15+00:00\",\"author\":{\"@id\":\"https:\/\/www.dentalcadmos.com\/#\/schema\/person\/dae8bc45e635361a55553f0aac0857fe\"},\"breadcrumb\":{\"@id\":\"https:\/\/www.dentalcadmos.com\/gingival-fibroblast-proliferation-and-interaction-with-different-nanohybrid-resin-based-composite-materials-an-in-vitro-study\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.dentalcadmos.com\/gingival-fibroblast-proliferation-and-interaction-with-different-nanohybrid-resin-based-composite-materials-an-in-vitro-study\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.dentalcadmos.com\/gingival-fibroblast-proliferation-and-interaction-with-different-nanohybrid-resin-based-composite-materials-an-in-vitro-study\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/www.dentalcadmos.com\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Gingival fibroblast proliferation and interaction with different nanohybrid resin-based composite materials: an in vitro study\"}]},{\"@type\":\"Person\",\"@id\":\"https:\/\/www.dentalcadmos.com\/#\/schema\/person\/dae8bc45e635361a55553f0aac0857fe\",\"name\":\"edra\",\"image\":{\"@type\":\"ImageObject\",\"@id\":\"https:\/\/www.dentalcadmos.com\/#personlogo\",\"inLanguage\":\"en-US\",\"url\":\"https:\/\/secure.gravatar.com\/avatar\/e2aad0a8340bb6125c47d6b52ca5828e?s=96&d=mm&r=g\",\"contentUrl\":\"https:\/\/secure.gravatar.com\/avatar\/e2aad0a8340bb6125c47d6b52ca5828e?s=96&d=mm&r=g\",\"caption\":\"edra\"},\"url\":\"https:\/\/www.dentalcadmos.com\/en\/author\/edra\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Gingival fibroblast proliferation and interaction with different nanohybrid resin-based composite materials: an in vitro study - Dental Cadmos","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.dentalcadmos.com\/en\/gingival-fibroblast-proliferation-and-interaction-with-different-nanohybrid-resin-based-composite-materials-an-in-vitro-study\/","og_locale":"en_US","og_type":"article","og_title":"Gingival fibroblast proliferation and interaction with different nanohybrid resin-based composite materials: an in vitro study - Dental Cadmos","og_description":"OBJECTIVES: To evaluate the interactions between human gingival fibroblasts (hGFs) and resin-based composite materials of different viscosities by analyzing fibroblast proliferation, adhesion and overall cellular response. MATERIALS AND METHODS: Two nanohybrid composite resins were tested: Estelite Universal Flow (flowable) and Estelite ASTERIA (packable). 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